期刊
ANALYTICAL BIOCHEMISTRY
卷 333, 期 2, 页码 225-229出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2004.05.013
关键词
capillary zone electrophoresis; mobility-charge relationship; protein charge; staphylococcal nuclease
资金
- Biotechnology and Biological Sciences Research Council [B18660] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [B18660] Funding Source: researchfish
The feasibility of employing classical electrophoresis theory to determine the net charge (valence) of proteins by capillary zone electrophoresis is illustrated in this paper. An outline of a procedure to facilitate the interpretation of mobility measurements is demonstrated by its application to a published mobility measurement for Staphylococcal nuclease at pH 8.9 that had been obtained by capillary zone electrophoresis. The significantly higher valence of +7.5 (cf. 5.6 from the same series of measurements) that has been reported on the basis of a charge ladder approach for charge determination signifies the likelihood that the latter generic approach may be prone to error arising from nonconformity of the experimental system with an inherent assumption that chemical modification or mutation of amino acid residues has no effect on the overall three-dimensional size and shape of the protein. (C) 2004 Elsevier Inc. All rights reserved.
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