期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1052, 期 1-2, 页码 1-11出版社
ELSEVIER
DOI: 10.1016/j.chroma.2004.07.110
关键词
gradient HPLC; ghost peaks; system peaks; chromatographic baselines; water; methanol; acetonitrile; solvents; TFA; artifacts
New examples and sources of ghost peaks in reversed-phase gradient HPLC are described and related to previous publications to give a broad perspective of ghost peak problems. In one new example the ghost peak was found to be due to mixing problems caused by a period of non-delivery of the stronger solvent B in a stepped gradient system. In a second example the ghost peak was due to plasticizer contamination of the organic solvent. This paper includes tips and recommendations for the consistent running of ghost-peak-free reversed-phase gradient HPLC. (C) 2004 Elsevier B.V. All rights reserved.
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