期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1053, 期 1-2, 页码 263-268出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2004.07.042
关键词
solid-phase extraction; on-line preconcentration; tee-split interface; peptides
Solid-phase extraction (SPE) and capillary electrophoresis (CE) are on-line coupled via a Tee-split interface, which provides hydrodynamic injection of the SPE eluate by flow splitting. The interface allows sample preconcentration independently from the CE separation and prevents sample matrix and washing solvents from entering the separation capillary. The effect of the Tee-split interface on the CE efficiency was examined using enkephalin peptides as model compounds. Most favorable plate numbers were obtained using a split ratio of 1:40. Breakthrough volume, desorption efficiency and elution volume for the C-18 micro SPE column (5 mm x 0.5 mm i.d.) were found to be 750 muL, 65% and 1 muL, respectively. The performance of the complete system was demonstrated by the preconcentration and separation of an enkephalin mixture. Plate numbers up to 120 000 were obtained using a sample volume of 250 muL and a split ratio of 1:40. Enkephalin peak areas were linear (R-2 = 0.996) over the 10-1000 ng/mL range. UV absorbance concentration limits of detection (S/N = 3) were about 5 ng/mL. For 250 muL injections of 100 ng/mL, the relative standard deviation (n = 5) of peak area was lower than 10%. (C) 2004 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据