期刊
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
卷 2004, 期 21, 页码 4323-4339出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ejoc.200400255
关键词
oligosaccharides; carbohydrate-carbohydrate recognition; gold glyconanoparticles; marine sponges
The first step in marine sponge cell recognition and adhesion operates via a calcium-dependent proteoglycan-proteoglycan interaction. For the marine sponge Microciona prolifera, one of the carbohydrate epitopes involved in the proteoglycan self-recognition is a sulfated disaccharide [GlcpNAc3S(beta1-3)Fucp]. Earlier surface plasmon resonance studies have demonstrated that the proteoglycan self-recognition can be mimicked with synthetic (beta-D-GlcpNAc-(1-->3)-alpha-L-Fucp-(1-->O), when multivalently presented by conjugation with bovine serum albumin. Here, the straightforward synthesis of water-soluble gold glyconanoparticles coated with the glycosides (beta-D-GlcpNAc3S-(1-->3)-alpha-L-Fucp(1-O)(CH2)(3)S(CH2)(6)SH, beta-D-GlcpNAc3S-(1-->3)-beta-L-Fucp-(1-->O)(CH2)(3)S(CH2)(6)SH, beta-D-GlcpNAc3S-(1-->O)(CH2)(3)S(CH2)(6)SH, alpha-L-Fucp-(1-->O)(CH2)(3)S(CH2)(6)SH, beta-D-GlcpNAc3S-(1-->3)-alpha-L-Galp-(1-->O)(CH2)(3)S(CH2)(6)SH, beta-D-GlcpNAc-(1-->3)-alpha-L-Fucp-(1-->O)(CH2)(3)S(CH2)(6)SH, and beta-D-Glcp3S-(1-->3)-alpha-L-Fucp-(1-->O)(CH2)(3)S(CH2)(6)SH is presented. Such supramolecular structures are excellent probes for studying carbohydrate-carbohydrate interactions by transmission electron microscopy, thereby generating information on the molecular level about the role of different functionalities in the self-recognition process. ((C) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据