Enhancement of rotifer (Brachionus plicatilis) growth by using terrestrial lactic acid bacteria

One of the pathways for the entry of bacteria, both pathogenic and probiotic, into the larvae of fish hatcheries is via live prey. As a preventive measure against infections, live prey may be cultured, supplementing the food with probiotics. Some lactic acid bacteria (LAB) have been successfully used in the larviculture. In this study, the nutritional effect of seven terrestrial LAB has been studied regarding the growth of the rotifer Brachionus plicatilis. The cultures were carried out without partial renewal of the culture medium, feeding the rotifers on baker's yeast and adding some of the species of bacteria. In all cases, the addition of the bacteria increased both the specific maximum growth rate and the maximum density obtainable in the cultures. However, the best results were obtained with the addition of Lactococcus casei ssp. casei, Pediococcus acidilactici, or Lactobacillus lactis spp lactis. The rates of growth obtained with the individual or joint addition of these three bacteria were 8-13 times greater than those obtained with the control cultures after 4-5 days' culture. In this study, a series of kinetic models has been applied (logistic modified-Gompertz, logistic-logistic, and generalised logistic) which describes the experimental data, obtaining a set of parameters of biological significance which facilitate the optimisation of the use of these bacterial strains in the mass production of rotifers. (C) 2004 Elsevier B.V. All rights reserved.