期刊
PROCESS BIOCHEMISTRY
卷 39, 期 12, 页码 2129-2133出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2003.11.004
关键词
biooxidation; ferrous iron; immobilization; packed-bed bioreactor; poly(vinyl alcohol); Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans was immobilized in poly(vinyl alcohol) (PVA) by a PVA-boric acid method, and spherical beads of uniform size were produced. Biooxidation of ferrous iron by immobilized cells was investigated in repeated batch culture and continuous operation in a laboratory scale packed-bed bioreactor. During repeated batch culture, the cell-immobilized gels were stable and showed high constant iron-oxidizing activity. In continuous operation in a packed-bed bioreactor, biooxidation of ferrous iron fits a plug-flow reaction model well. A maximum Fe2+ oxidation rate of 1.89g l(-1) h(-1) was achieved at the dilution rate of 0.38h(-1) or higher, while no obvious precipitate was detected in the bioreactor. (C) 2003 Elsevier Ltd. All rights reserved.
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