Influence of season and environment on adult neurogenesis in the central olfactory pathway of the shore crab, Carcinus maenas

In most vertebrates hitherto examined including humans, certain brain areas retain the capacity to build new neurons during adult life. In some arthropods, above all in crustaceans, continuous genesis of brain neurons has also been shown, namely for soma clusters of the olfactory brain. Several factors as, e.g., sensory input, living conditions, or stress, are known to influence the rate of cell proliferation, survival, and cell differentiation. The present study was undertaken to test whether seasonal changes and/or captivity would influence the proliferation of cells in the lateral cluster (LC) of the olfactory lobe (OL) and in the cluster of the hemiellipsoid body (HB) of the eyestalk of shore crabs. During a period of more than a year, 5-bromo-deoxyuridine (BrdU) injections were administered to freshly caught animals and to animals kept for 12 weeks after capture under artificial conditions. Counts of BrdU-labeled cells showed that animal size, seasonal changes as well as captivity had an influence on the number of proliferating cells. Further, in the lateral soma cluster and the soma cluster of the hemiellipsoid body, cell proliferation is most likely regulated independently. While the lateral soma cluster showed two peaks of cell proliferation (spring and late summer), the soma cluster of the hemiellipsoid body had only one peak in early summer. Furthermore, proliferation decreased with size and hence age of the animal only in the lateral soma cluster but not in the soma cluster of the hemiellipsoid body. Although captivity reduced the number of newborn cells in general, cell proliferation remained synchronous with the seasons of the year, indicating that an endogenous circannual rhythm regulates neurogenesis. (C) 2004 Elsevier B.V. All rights reserved.