Evaluation of a methylation procedure to determine cyclopropenoids fatty acids from Sterculia striata St. Hil. Et Nauds seed oil

Cyclopropenoids fatty acids (CPFA) from Sterculia striata seed oil were characterized by gas chromatography/mass spectrometry (GC/MS) and quantified by gas chromatography-flame ionization detector (GC-FID) after derivation to fatty acid methyl esters using a cold base-catalyzed procedure. H-1 nuclear magnetic resonance (NMR) analysis were done in oil and fatty acid methyl esters derivatives to quantify CPFA and verify artifacts formation during the base-catalyzed reaction. Similar quantities of CPFA were found in S. striata and Sterculia foetida seed oils before and after a base-catalyzed methylation by NMR analysis, with no artifact fort-nation. These results were compatible with those obtained by GC-FID analysis. Transmethylation with KOH in methanol was an appropriated method to prepare cyclopropenoids fatty acids methyl esters and quantify them by GC and NMR analysis. (C) 2004 Elsevier B.V. All rights reserved.