期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 139, 期 2, 页码 153-159出版社
ELSEVIER
DOI: 10.1016/j.jneumeth.2004.04.020
关键词
ELISA; PSD-95; post-mortem delay; synaptophysin; syntaxin
资金
- Alzheimers Research UK [ART-PG2002-3] Funding Source: researchfish
Assessments of synaptic density in human brain are often based on measurements of synaptic proteins. Little information is available on their post-mortem stability. We have investigated this by ELISAs of the pre-synaptic proteins syntaxin and synaptophysin, and the post-synaptic protein PSD-95, in rat and human cortex. The rat brains were cooled in situ from 37 to 20 or 4degreesC over 3 h, and then kept at 20 or 4degreesC for a further 24-72 h, to simulate post-mortem storage at room temperature or in a mortuary refrigerator. Synaptophysin and PSD-95 levels in rat cerebral cortex were not significantly decreased after 72 h of incubation at 20degreesC. Syntaxin was stable for 24 h but decreased by 39-44% at 48-72 h. Storage at 4degreesC resulted in a similar reduction of syntaxin levels over 72 h. In human brain tissue from 160 people aged 24-102 years, post-mortem delay had little effect on synaptic protein levels in superior temporal cortex, but was associated with a decline in PSD-95 and syntaxin in mid-frontal cortex after 24 h. The more robust stability of synaptophysin may be related to its multi-transmembrane structure. (C) 2004 Elsevier B.V. All rights reserved.
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