期刊
APPLIED PHYSICS LETTERS
卷 98, 期 9, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.3554415
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资金
- UK EPSRC
- Royal Society
- MRC
- SUPA
- EPSRC [EP/D048958/1, EP/G029733/1] Funding Source: UKRI
- MRC [G0802645] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/D048958/1, EP/G029733/1] Funding Source: researchfish
- Medical Research Council [G0802645] Funding Source: researchfish
We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique. (C) 2011 American Institute of Physics. [doi:10.1063/1.3554415]
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