期刊
PHYTOCHEMISTRY
卷 65, 期 21, 页码 2885-2891出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2004.07.024
关键词
monoamine oxidase A; human recombinant; GST-fusion protein; HPLC; activity profiling; plant extract; lead discovery
An assay for the HPLC-based search for monoamine oxidase-A (MAO-A) inhibitors in plant extracts was established. It combines human recombinant MAO-A, expressed as GST-fusion protein in yeast, with a kinetic measurement of the conversion of kynuramine to 4-hydroxyquinoline. Substrate selectivity and kinetic parameters of the GST-fusion protein were comparable to the wild-type enzyme. The applicability of the assay to HPLC-based activity profiling was tested with plant extracts spiked with small amounts of known MAO inhibitors. (C) 2004 Elsevier Ltd. All rights reserved.
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