期刊
CHEMISTRY & BIOLOGY
卷 11, 期 11, 页码 1583-1594出版社
CELL PRESS
DOI: 10.1016/j.chembiol.2004.09.004
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资金
- NIAID NIH HHS [T32 AI07254] Funding Source: Medline
A small library of pyrrole-imidazole polyamide-DNA alkylator (chlorambucil) conjugates was screened for effects on morphology and growth characteristics of a human colon carcinoma cell line, and a compound was identified that causes cells to arrest in the G2/M stage of the cell cycle. Microarray analysis indicates that the histone Hoc gene is significantly downregulated by this polyamide. RT-PCR and Western blotting experiments confirm this result, and siRNA to Hoc mRNA yields the same cellular response. Strikingly, reduction of H4 protein by >50% does not lead to widespread changes in global gene expression. Sequence-specific alkylation within the coding region of the Hoc gene in cell culture was confirmed by LM-PCR. The compound is active in a wide range of cancer cell lines, and treated cells do not form tumors in nude mice. The compound is also active in vivo, blocking tumor growth in mice, without obvious animal toxicity.
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