期刊
EXPERIMENTAL CELL RESEARCH
卷 300, 期 2, 页码 345-353出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2004.07.025
关键词
Saccharomyces cerevisiae; yeast; apoptosis; TUNEL; reactive oxygen species; vacuolc; starvation; stationary phase; cellular stress; rho(0)
Protracted starvation of auxotrophic Saccharomyces cerevisiae strains for an essential amino acid is commonly used to allow investigation of adaptive mutation mechanisms during starvation-induced cell cycle arrest. Under these conditions, the majority of cells dies during the first 6 days. We investigated starving cells for markers of programmed cell death and for the production of reactive oxygen species (ROS). We observed that protracted starvation for lysine or histidine resulted in an increasing number of cells exhibiting DNA fragmentation and chromatin condensation, thus an apoptotic phenotype. Not only respiration-competent cells but also respiratory deficient rho 0 cells were able to undergo programmed cell death. In addition the starving cells rapidly exhibited indicators of oxidative stress, independently of their respiratory competence. These results indicate that starvation for an essential amino acid results in severe cell stress, which may finally be the trigger of programmed cell death. (C) 2004 Elsevier Inc. All rights reserved.
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