期刊
PHYTOCHEMISTRY
卷 65, 期 21, 页码 2893-2896出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2004.08.052
关键词
Crinum glaucum; Crinum jagus; Amaryllidaceae; alkaloids; acetylcholinesterase inhibition; Alzheimer's disease
The bulbs of Crinum jagus and Crinum glaucum are used in traditional medicine in southern Nigeria for memory loss and other mental symptoms associated with ageing. Alkaloidal extracts of bulbs from each species showed inhibition of acetylcholinesterase, an activity exploited therapeutically to raise the depressed levels of acetylcholine in the brain associated with Alzheimer's disease. Using the in situ bioautographic test method for enzyme inhibition, a number of alkaloids were isolated and their activity quantified using the Ellman spectrophotometric test. The most active alkaloids isolated were hamayne (IC50 250 muM) and lycorine (IC50 450 muM) whilst other alkaloids were comparatively inactive with haernanthamane giving 3degreesC inhibition and crinamine giving 4.4% inhibition at 50 mg ml(-1) (174 muM). These contrast with the positive control physostigmine which gave IC50 of 0.25 muM. Cholinesterase activity appears to be associated with the presence of two free hydroxy groups in this structural type of Amaryllidaceae alkaloid. (C) 2004 Elsevier Ltd. All rights reserved.
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