期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 70, 期 11, 页码 6887-6891出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.70.11.6887-6891.2004
关键词
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A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The plasmid was used in Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus to efficiently construct mutants with or without an associated antibiotic resistance gene.
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