期刊
FREE RADICAL RESEARCH
卷 38, 期 11, 页码 1223-1233出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10715760400017376
关键词
dicumarol; radiation; reactive oxygen species; manganese; superoxide dismutase; pancreatic cancer
Manganese superoxide dismutase (MnSOD) levels have been found to be low in human pancreatic cancer [ Pancreas 26, ( 2003), 23] and human pancreatic cancer cell lines [ Cancer Res. 63, ( 2003), 1297] when compared to normal human pancreas. We hypothesized that stable overexpression of pancreatic cancer cells with MnSOD cDNA would alter the malignant phenotype. MIA PaCa-2 cells were stably transfected with a pcDNA3 plasmid containing sense human MnSOD cDNA or containing no MnSOD insert by using the lipofectAMINE method. G418-resistant colonies were isolated, grown and maintained. Overexpression of MnSOD was confirmed in two selected clones with a 2-4-fold increase in MnSOD immunoreactive protein. Compared with the parental and neo control cells, the MnSOD-overexpressing clones had decreased growth rates, growth in soft agar and plating efficiency in vitro, while in vivo, the MnSOD-overexpressing clones had slower growth in nude mice. These results suggest that MnSOD may be a tumor suppressor gene in human pancreatic cancer.
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