期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
卷 168, 期 1-2, 页码 67-73出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2004.02.014
关键词
anthraquinones; photosensitisation; singlet oxygen; superoxide anion; spin trapping; DNA strand break; EPR
Photosensitising properties of four naturally occurring anthraquinones, viz., erythroglaucin (ERG), teloschistin (TEL), 1-hydroxy-2-methylanthraquinone (HYQ) and 1-methoxy-2-hydroxyanthraquinone (MEQ) are studied. Photogeneration of singlet oxygen was monitored by both optical RNO (para-nitrosodimethylaniline) bleaching and electron paramagnetic resonance spectroscopy (EPR)-2,2,6,6-tetramethyl piperidinol (TEMPL) methods. In comparison with rose bengal (RB), singlet oxygen generating efficiencies of ERG, TEL, HYQ and MEQ are determined to be 0.10, 0.14, 0.02 and 0.13, respectively. The formation of O-2(.-) was monitored by optical spectroscopy using SOD inhibitable cytochrome c reduction assay. Photolysis of ERG and MEQ in DMSO, in the presence of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) generated a multiline EPR spectrum characteristic of mixture of O-2(.-), (OH)-O-. and (OCH3)-O-. adducts. Both electron transfer (Type 1) and energy transfer (Type II) paths are involved in the photosensitisation of ERG, TEL, HYQ and MEQ. Quantum efficiencies of the quinones exhibit correlation with their half-wave potentials. The photoinduced DNA cleavage by quinones was examined with covalently closed circular plasmid DNA, in vitro. Observed DNA photo strand scission correlates with the reactive oxygen species (ROS) generating efficiencies of the quinones. (C) 2004 Elsevier B.V. All rights reserved.
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