期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 44, 页码 24864-24875出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b08124
关键词
protein mobility; cation immobilization; anionic polysaccharide; surface modification; paper-based analytical device
资金
- Japan Agency for Medical Research and Development
- AMED
- Graduate School of Science and Technology, Keio University
Distance-based detection motifs on microfluidic paper-based analytical devices (mu PADs) allow quantitative analysis without using signal readout instruments in a similar manner to classical analogue thermometers. To realize a cost-effective and calibration-free distance-based assay of lactoferrin in human tear fluid on a mu PAD not relying on antibodies or enzymes, we investigated the fluidic mobilities of the target protein and Tb3+ cations used as the fluorescent detection reagent on surface-modified cellulosic filter papers. Chromatographic elution experiments in a tear-like sample matrix containing electrolytes and proteins revealed a collapse of attractive electrostatic interactions between lactoferrin or Tb3+ and the cellulosic substrate, which was overcome by the modification of the paper surface with the sulfated polysaccharide iota-carrageenan. The resulting mu PAD based on the fluorescence emission distance successfully analyzed 0-4 mg mL(-1) of lactoferrin in complex human tear matrix with a lower limit of detection of 0.1 mg mL(-1) by simple visual inspection. Assay results of 18 human tear samples including ocular disease patients and healthy volunteers showed good correlation to the reference ELISA method with a slope of 0.997 and a regression coefficient of 0.948. The distance-based quantitative signal and the good batch-to-batch fabrication reproducibility relying on printing methods enable quantitative analysis by simply reading out concentration scale marks printed on the mu PAD without performing any calibration and using any signal readout instrument.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据