期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 42, 期 11, 页码 5327-5331出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.42.11.5327-5331.2004
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A nucleotide transversion from guanine to uracil in the 23S rRNA confers linezolid resistance. We describe a real-time PCR using two Taqman probes that detects a single mutated allele among the genomes of Enterococcus faecium and Enterococcus faecalis. Results were confirmed by a classical approach involving LabChip technology assayed with an Agilent Bioanalyzer 2100.
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