期刊
EXPERT OPINION ON BIOLOGICAL THERAPY
卷 4, 期 11, 页码 1821-1829出版社
TAYLOR & FRANCIS LTD
DOI: 10.1517/14712598.4.11.1821
关键词
biopharmaceuticals; cell culture; flow cytometry; gel microdrop; high producers; matrix-based secretion assays
The selection of high-producing cell lines is usually time-consuming and labour-intensive. Following transfection, high-producing cells are selected using limiting dilution cloning to prevent non- and low-producing cells from outgrowing high-producing cells, a process that normally takes > 3 months. During this time, the cells have to be screened occasionally to ensure stability of the selected clone. Several new methods for selecting and screening cells using flow cytometry and cell sorting have recently been developed; these include gel microdrop technology, which encapsulates the cells in gelatine beads, and matrix-based secretion assays. This paper reviews these techniques for selecting high-producing cell lines and isolating rare cells.
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