期刊
JOURNAL OF NEUROSCIENCE
卷 24, 期 44, 页码 9897-9902出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2705-04.2004
关键词
voltage-sensitive dye; tangential slice; optical imaging; oscillation; partial differential equations; spiral waves
资金
- NIMH NIH HHS [K02 MH001493, K02MH01493] Funding Source: Medline
- NINDS NIH HHS [R01 NS036447, R01NS036447, R01 NS036447-06] Funding Source: Medline
Spiral waves are a basic feature of excitable systems. Although such waves have been observed in a variety of biological systems, they have not been observed in the mammalian cortex during neuronal activity. Here, we report stable rotating spiral waves in rat neocortical slices visualized by voltage-sensitive dye imaging. Tissue from the occipital cortex (visual) was sectioned parallel to cortical lamina to preserve horizontal connections in layers III-V (500-mum-thick, similar to4 x 6 mm(2)). In such tangential slices, excitation waves propagated in two dimensions during cholinergic oscillations. Spiral waves occurred spontaneously and alternated with plane, ring, and irregular waves. The rotation rate of the spirals was similar to10 turns per second, and the rotation was linked to the oscillations in a one-cycle-one-rotation manner. A small (<128 mu m) phase singularity occurred at the center of the spirals, about which were observed oscillations of widely distributed phases. The phase singularity drifted slowly across the tissue (similar to 1 mm/10 turns). We introduced a computational model of a cortical layer that predicted and replicated many of the features of our experimental findings. We speculate that rotating spiral waves may provide a spatial framework to organize cortical oscillations.
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