期刊
SCIENCE
卷 306, 期 5698, 页码 1046-1048出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1101092
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资金
- Biotechnology and Biological Sciences Research Council [BBS/E/J/00000594] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BBS/E/J/00000594] Funding Source: researchfish
In RNA interference (RNAi), double-stranded RNA (dsRNA) triggers degradation of homologous messenger RNA. In many organisms, RNA-dependent RNA polymerase (RdRp) is required to initiate or amplify RNAi, but the substrate for dsRNA synthesis in vivo is not known. Here, we show that RdRp-dependent transgene silencing in Arabidopsis was caused by mutation of XRN4, which is a ribonuclease (RNase) implicated in mRNA turnover by means of decapping and 5'-3' exonucteotysis. When both XRN4 and the RdRp were mutated, the plants accumulated decapped transgene mRNA. We propose that mRNAs lacking a cap structure become exposed to RdRp to initiate or maintain RNAi.
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