期刊
JOURNAL OF NEUROSCIENCE
卷 24, 期 45, 页码 10201-10210出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3246-04.2004
关键词
insulin-like growth factor-I; IGF-I; mitosis; neurogenesis; transgenic mice; ventricular zone
资金
- NICHD NIH HHS [HD08299, R01 HD008299] Funding Source: Medline
Neurogenesis in the developing cerebral cortex of mice occurs in the dorsal telencephalon between embryonic day 11 ( E11) and E17, during which time the majority of cortical projection neurons and some glia are produced from proliferating neuroepithelial cells in the ventricular zone. The number of cells produced by this process is governed by several factors, including cell cycle kinetics and the proportion of daughter cells exiting the cell cycle after a given round of cell division. The in vivo effects of IGF-I on cell cycle kinetics were investigated in nestin/IGF-I transgenic ( Tg) embryos, in which IGF-I is overexpressed in the cerebral cortex and dorsal telencephalon. These Tg mice have been shown to exhibit increased cell number in the cortical plate by E16 and increased numbers of neurons and glia in the cerebral cortex during postnatal development. Cumulative S phase labeling with 5-bromo-2'-deoxyuridine revealed a decrease in total cell cycle length ( T-C) in Tg embryos on E14. This decrease in T-C was found to result entirely from a reduction in the length of the G(1) phase of the cell cycle from 10.66 to 8.81 hr, with no significant changes in the lengths of the S, G(2), and M phases. Additionally, the proportion of daughter cells reentering the cell cycle was significantly increased by 15% in Tg embryos on E14-E15 compared with littermate controls. These data demonstrate that IGF-I regulates progenitor cell division in the ventricular zone by reducing G(1) phase length and decreasing T-C but increases cell cycle reentry.
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