期刊
TALANTA
卷 64, 期 4, 页码 1018-1023出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2004.04.022
关键词
miniaturization; capillary electrophoresis; amperometric detection; carbon nanotube; homocysteine; cysteine; glutathione; N-acetylcysteine
Multi-walled carbon nanotube (CNT) was mixed with epoxy to fabricate microdisc electrode used as a detector for a specially designed miniaturized capillary electrophoresis (CE)-amperometric detection system for the separation and detection of several bioactive thiols. The end-channel CNT amperometric detector offers favourable signal-to-noise characteristics at a relatively low potential (0.8 V) for detecting thiol compounds. Factors influencing the separation and detection processes were examined and optimized. Four thiols (homocysteine, cysteine, glutathione, and N-acetylcysteine) have been separated within 130 s at a separation voltage of 2000 V using a 20 mM phosphate running buffer (pH 7.8). Highly linear response is obtained for homocysteme, cysteine, glutathione, and N-acetylcysteine over the range of 5-50 muM with detection limits of 0.75, 0.8, 2.9, and 3.3 muM, respectively. Good stability and reproducibility (R.S.D. < 5%) are obtained reflecting the minimal adsorption of thiols at the CNT electrode surface. The new microchip protocol should find a wide range of bioanalytical applications involving assays of thiol compounds. (C) 2004 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据