First observations of predation by New Zealand Greenshell mussels (Perna canaliculus) on zooplankton

Observations made overseas of predation by blue mussels and zebra mussels on mesozooplankton (>200 mun) have raised concern within New Zealand that the Greenshell mussel, Perna canaliculus, which is cultured in large tonnages throughout hundreds of marine farms within the New Zealand coastal zone, could exert ecologically detrimental effects by preying on zooplankton. We conducted experiments at Clova Bay, Pelorus Sound in May 2002 to determine the rates that P. canaliciulus infests prey, tip to and including the mesozooplankton size range. Single mussels from farms were incubated with seawater enriched with zooplankton (>60 mum) in gently circulated 15-1 pails. Depletion of chlorophyll-a (chl-a), ciliate microzooplankton, and nauplii, copepodites, and adults of copepods was determined over 5 h, relative to controls with no mussels. Two experiments were made over consecutive days. Gut contents of these experimental mussels, and of mussels examined soon after collection from a farm, were described. Gut contents of experimental and of freshly collected mussels (standard shell length similar to 90 mm) had numerous copepod parts, whole copepods and larval bivalves present. Experimental mussels cleared chl-a and ciliates from 59- to 137-1 individual(-1) day(-1), respectively, averaged across the two experiments. Faster ciliate than chl-a clearance was probably caused by the high proportion (56%) of phytoplankton below the retention size for R canaliculus (ca. 5 mum) and by faster ciliate grazing in controls than treatments. The average clearance rates of adult, copepodite, and naupliar copepod stages by mussels were 20, 31, and 49 1 individual(-1) day(-1), respectively. The clearance rates of each copepod stage were not significantly different between the two experiments. Clearance of nauplii was significantly greater than of adults and copepodites, while adult and copepodite clearance rates were nearly significantly different. The mean lengths of the adult, copepodite, and naupliar copepods were 430, 265, and 165 mum, respectively. The decreasing clearance rates with increasing size and development of prey (from ciliates, through naupliar, copepodite to adult copepods), suggested that prey escape ability, related to body size and/or morphology, affected capture rates. Mussel faecal samples indicated complete digestion of the gut contents. Pseudofaecal samples showed very low rejection rates of mesozooplankton by mussels. The results are considered in context of current biophysical modelling studies of impacts of large mussel farms in New Zealand. Designs of future experiments to improve accuracy of estimates of mesozooplankton clearance rates by P canaliculus are considered. (C) 2004 Elsevier B.V. All rights reserved.