期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 279, 期 47, 页码 48865-48875出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M409699200
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资金
- NHLBI NIH HHS [HL56593] Funding Source: Medline
The discovery of autosomal dominant hypercholesterolemic patients with mutations in the PCSK9 gene, encoding the proprotein convertase NARC-1, resulting in the missense mutations suggested a role in low density lipoprotein (LDL) metabolism. We show that the endoplasmic reticulum-localized proNARC-1 to NARC-1 zymogen conversion is Ca2+-independent and that within the zymogen autocatalytic processing site SS (V) under bar FA (Q) under bar down arrowSI (P) under bar Val at P4 and Pro at P3' are critical. The S127R and D374Y mutations result in similar to50-60% and greater than or equal to98% decrease in zymogen processing, respectively. In contrast, the double [D374Y+N157K], F216L, and R218S natural mutants resulted in normal zymogen processing. The cell surface LDL receptor (LDLR) levels are reduced by 35% in lymphoblasts of S127R patients. The LDLR levels are also reduced in stable HepG2 cells overexpressing NARC-1 or its natural mutant S127R, and this reduction is abrogated in the presence of 5 mM ammonium chloride, suggesting that overexpression of NARC-1 increases the turnover rate of the LDLR. Adenoviral expression of wild type human NARC-1 in mice resulted in a maximal similar to9-fold increase in circulating LDL cholesterol, while in LDLR(-/-) mice a delayed similar to2-fold increase in LDL cholesterol was observed. In conclusion, NARC-1 seems to affect both the level of LDLR and that of circulating apoB-containing lipoproteins in an LDLR-dependent and -independent fashion.
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