Detection of template binding to molecularly imprinted polymers by Raman microspectroscopy

We report on sensitive and specific detection and quantification of a template in a molecularly imprinted polymer (MIP) using Raman microspectroscopy. The beta-blocking drug S-propranolol and its enantiomer, R-propranolol, were used as target molecules since the selectivity of this MIP is well established and serves as an appropriate validation standard. Specific peaks originating in the template were identified in the Raman spectrum, allowing quantification of bound target molecule. We demonstrate that label-free monitoring can be achieved from volumes as small as 1 mu m(3) of MIP, based on a single identifying peak.