Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection

Cerium oxide nanoparticles (NanoCeO(2)) and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of NanoCeO(2) increases effective electro-active surface area of CH-NanoCeO(2)/indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. BSA/r-IgGs/CH-NanoCeO(2)/ITO immunoelectrode exhibits improved linearity (0.25-6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity (18 mu A/ng dl(-1) cm(-2)), and regression coefficient (r(2) similar to 0.997). (C) 2009 American Institute of Physics. [doi: 10.1063/1.3249586]