期刊
MECHANISMS OF DEVELOPMENT
卷 121, 期 12, 页码 1455-1468出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mod.2004.08.001
关键词
sticks-and-stones; sns; dumbfounded; duf; kin of irreC; kirre; irregular chiasm C; irreC; roughest; rst; cell adhesion; myoblast fusion; immunoglobulin superfamily; schneider cells
资金
- NIAMS NIH HHS [R01 AR44274] Funding Source: Medline
The body wall muscles in the Drosophila larva arise from interactions between Duf/Kirre and Irregular chiasm C-roughest (IrreC-rst)expressing founder myoblasts and sticks-and-stones (SNS)-expressing fusion competent myoblasts in the embryo. Herein, we demonstrate that SNS mediates heterotypic adhesion of S2 cells with Duf/Kirre and IrreC-rst-expressing S2 cells, and colocalizes with these proteins at points of cell contact. These properties are independent of their transmembrane and cytoplasmic domains, and are observed quite readily with GPI-anchored forms of the ectodomains. Heterotypic interactions between Duf/Kirre and SNS-expressing S2 cells occur more rapidly and to a greater extent than homotypic interactions with other Duf/Kirre-expressing cells. In addition, Duf/Kirre and SNS are present in an immunoprecipitable complex from S2 cells. In the embryo, Duf/Kirre and SNS are present at points of contact between founder and fusion competent cells. Moreover, SNS clustering on the cell surface is dependent on Duf/Kirre and/or IrreC-rst. Finally, although the cytoplasmic and transmembrane domains of SNS are expendable for interactions in culture, they are essential for fusion of embryonic myoblasts. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
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