期刊
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
卷 118, 期 6, 页码 361-363出版社
SPRINGER
DOI: 10.1007/s00414-004-0473-0
关键词
ACTBP2; mutation; primer binding region; multiplex; database
In the course of routine genotyping of forensic reference samples by multiplex PCR, an allelic drop-out due to mutations in the primer binding regions of the highly polymorphic STR marker ACTBP2 was observed in 17 samples. The variation rate was estimated to be 0.0014 (95% confidence interval: 0.0006 - 0.003). The most frequently found mutation was an G to A transition in the reverse primer binding region which was present in 14 out of 17 cases. To overcome the problem we have added a modified reverse primer to different multiplex kits that led to the correct genotype.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据