期刊
POULTRY SCIENCE
卷 83, 期 12, 页码 2083-2085出版社
ELSEVIER
DOI: 10.1093/ps/83.12.2083
关键词
egg products; PCR; species identification
Species-specific primers for duck were deduced from the mitochondrial ATPase8 gene sequence. Species-specific PCR for turkeys and ducks showed no cross reaction with mixtures from chicken and guinea eggs and detection was possible to a concentration of 0.1% of homogenized duck egg and 5% of homogenized turkey egg when a PCR of 35 cycles was applied. A PCR of 30 cycles detected 10% of homogenized duck egg. The same sensitivities were obtained in dilutions of homogenized egg yolk; however, no PCR signals were obtained in egg white. Analysis of 13 industrial egg product samples showed the practical relevance of the species-specific PCR tests described herein.
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