4.5 Article Proceedings Paper

Batch and continuous fermentation of succinic acid from wood hydrolysate by Mannheimia succiniciproducens MBEL55E

期刊

ENZYME AND MICROBIAL TECHNOLOGY
卷 35, 期 6-7, 页码 648-653

出版社

ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2004.08.018

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Mannheimia succiniciproducens MBEL55E; succinic acid; wood hydrolysate; xylose

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Batch and continuous cultures of Mannheimia succiniciproducens MBEL55E were carried out in a complex medium containing a NaOH-treated wood hydrolysate for the production of succinic acid. The wood hydrolysate based medium was treated with NaOH before sterilization to reduce the formation of inhibitory compounds. M. succiniciproducens MBEL55E utilized xylose as well as glucose in the wood hydrolysate based medium as a carbon source for the succinic acid production. In batch cultures, the final succinic acid concentration of 11.73 g1(-1) was obtained from the pre-treated wood hydrolysate based medium, resulting in a succinic acid yield of 56% and a succinic acid productivity of 1.17 g1(-1) h(-1), while the corresponding continuous cultures gave the succinic acid yield and productivity of 55% and 3.19 g1(-1) h(-1), respectively. These results suggest that succinic acid can be produced economically and efficiently by the fermentation of M. succiniciproducens MBEL55E from an inexpensive biomass-based wood hydrolysate. (C) 2004 Elsevier Inc. All rights reserved.

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