期刊
MICROSCOPY RESEARCH AND TECHNIQUE
卷 65, 期 6, 页码 292-294出版社
WILEY-LISS
DOI: 10.1002/jemt.20132
关键词
lectin cytochemistry; confocal microscopy; fluorescence microscopy; image processing; human peripheral blood
This article presents a method for identification and localization of cell surface and intracellular sialoglycoconjugates of peripheral blood cells. To reveal cell surface conjugates, a sample of peripheral blood was incubated with lectin after centrifugation and rinsing. For intracellular localization in leukocytes, RBCs were lysed and the membranes were permeabilized prior to cytochemical reaction. Fluorescein isothiocyanate conjugated lectins were used for visualization in fluorescence microscope. All lectins bound specifically to the surface of erythrocytes. Confocal microscopy showed surface and intracellular labeling of permeabilized leukocytes. A part of the signal in eosinophils originated from binding of anionic fluorophore to cationic granular proteins. (C) 2005 Wiley-Liss, Inc.
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