期刊
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
卷 287, 期 6, 页码 F1179-F1188出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajprenal.00211.2004
关键词
anion exchanger; extracellular volume; blood pressure; HTA; acid-base
资金
- NIDDK NIH HHS [DK-33793, DK-17433, R37 DK033793] Funding Source: Medline
Pendrin ( Pds; Slc26A4) is a new anion exchanger that is believed to mediate apical Cl-/HCO3- exchange in type B and non-A-non- B intercalated cells of the connecting tubule and cortical collecting duct. Recently, it has been proposed that this transporter may be involved in NaCl balance and blood pressure regulation in addition to its participation in the regulation of acid-base status. The purpose of our study was to determine the regulation of Pds protein abundance during chronic changes in chloride balance. Rats were subjected to either NaCl, NH4Cl, NaHCO3, KCl, or KHCO3 loading for 6 days or to a low-NaCl diet or chronic furosemide administration. Pds protein abundance was estimated by semiquantitative immunoblotting in renal membrane fractions isolated from the cortex of treated and control rats. We observed a consistent inverse relationship between Pds expression and diet-induced changes in chloride excretion independent of the administered cation. Conversely, NaCl depletion induced by furosemide was associated with increased Pds expression. We conclude that Pds expression is specifically regulated in response to changes in chloride balance.
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