期刊
JOURNAL OF INVESTIGATIVE DERMATOLOGY
卷 123, 期 6, 页码 1176-1181出版社
ELSEVIER SCIENCE INC
DOI: 10.1111/j.0022-202X.2004.23485.x
关键词
cutaneous wound healing; fibronectin; keratinocytes; integrin alpha 9 beta 1
类别
资金
- NCI NIH HHS [R01 CA-17007] Funding Source: Medline
- NEI NIH HHS [R01 EY-08512] Funding Source: Medline
- NHLBI NIH HHS [P01 HL-41484] Funding Source: Medline
- NIA NIH HHS [P60 AG10415] Funding Source: Medline
- NIGMS NIH HHS [R01 GM056442, R01 GM-56442] Funding Source: Medline
The fibronectins (FN) comprise a family of adhesive extracellular matrix proteins thought to mediate important functions in cutaneous wounds. Plasma fibronectin (pFN) extravasates for days from intact hyperpermeable vessels following injury whereas mRNAs encoding the cellular fibronectins (cFN) that include two segments, termed EIIIA (EDA) and EIIIB (EDB), are expressed by wound cells. Wounds in mice null for pFN appear to heal normally whereas those in EIIIA null mice exhibit defects, suggesting that cFN may play a role when pFN is missing. Integrin alpha 9 beta 1, a receptor for several extracellular matrix proteins as well as the EIIIA segment, is expressed normally in the basal layer of squamous epithelia. We report results from immunohistochemistry on healing wounds demonstrating that EIIIA-containing cFN are deposited abundantly but transiently from day 4 to 7 whereas EIIIB-containing cFN persist at least through day 14. Elevated expression of alpha 9 beta 1 is seen in basal and suprabasal epidermal keratinocytes in wounds. The spatial expression patterns of cFN and alpha 9 beta 1 are distinct, but overlap in the dermal-epidermal junction, and both are expressed contemporaneously. These observations suggest a role for alpha 9 beta 1-EIIIA interactions in wound keratinocyte function.
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