期刊
JOURNAL OF NEUROCHEMISTRY
卷 91, 期 5, 页码 1180-1190出版社
WILEY
DOI: 10.1111/j.1471-4159.2004.02793.x
关键词
A(2B) adenosine receptors; cerebral damage; cytokines; functional cross-talk; human astrocytoma cells; reactive astrogliosis
Low-affinity A(2B) adenosine receptors (A(2B) ARs), which are expressed in astrocytes, are mainly activated during brain hypoxia and ischaemia, when large amounts of adenosine are released. Cytokines, which are also produced at high levels under these conditions, may regulate receptor responsiveness. In the present study, we detected A(2B) AR in human astrocytoma cells (ADF) by both immunoblotting and real-time PCR. Functional studies showed that the receptor stimulated adenylyl cyclase through Gs proteins. Moreover, A(2B) ARs were phosphorylated and desensitized following stimulation of the receptors with high agonist concentration. Tumour necrosis factor alpha (TNF-alpha) treatment (24- h) increased A(2B) AR functional response and receptor G protein coupling, without any changes in receptor protein and mRNA levels. TNF-alpha markedly reduced agonist-dependent receptor phosphorylation on threonine residues and attenuated agonist-mediated A(2B) ARs desensitization. In the presence of TNF-alpha, A(2B) AR stimulation in vitro induced the elongation of astrocytic processes, a typical morphological hallmark of in vivo reactive astrogliosis. This event was completely prevented by the selective A(2B) AR antagonist MRS 1706 and required the presence of TNF-alpha. These results suggest that, in ADF cells, TNF-alpha selectively modulates A(2B) AR coupling to G proteins and receptor functional response, providing new insights to clarify the pathophysiological role of A(2B) AR in response to brain damage.
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