期刊
SOIL SCIENCE AND PLANT NUTRITION
卷 50, 期 8, 页码 1273-1276出版社
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION
DOI: 10.1080/00380768.2004.10408603
关键词
microarray; quantitative reverse transcription mediated-PCR; rice; silicon; transcript
Rice plants were grown for 4 weeks in nutrient solutions containing 0, 0.015, 0.15, and 1.5 mm silicate. Si content in leaves was lower in the plants grown at low silicate concentrations. RNA was extracted from leaves and subjected to microarray analysis. Statistical analysis of the data from two independent hybridizations enabled to identify a number of candidate clones regulated by Si nutritional conditions. Quantitative reverse transcriptionmediated-PCR enabled to identify five genes whose transcript accumulation was affected by Si nutrition.
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