期刊
CHEMBIOCHEM
卷 5, 期 12, 页码 1686-1691出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.200400080
关键词
electrochemistry; electrodes; myoglobin; nanoparticles
Silver nanoparticles (11 +/- 1.5 nm) could greatly enhance the electron-transfer reactivity of myoglobin (Mb) and its catalytic ability toward hydrogen peroxide (H2O2). Direct fast electron transfer between Mb and a pyrolytic graphite (PG) electrode was achieved, and a pair of well-defined, quasireversible redox peaks was obtained. The cathodic and anodic peaks were located at -329 and -281 mV, respectively. Meanwhile, the catalytic ability of the protein toward the reduction of H2O2 was also studied, and a H2O2 biosensor was subsequently fabricated. Its detection limit Was 1.0 x 10(-6) M with a sensitivity of 0.0205 muA per muM of H2O2, The apparent Michaelis-Menten constant was calculated to be 1303 muM. Flocculation assay showed that the protein maintained plasmon layers surrounding the surface of silver nonoparticles and avoided silver-nanoparticle aggregation. On the other hand, UV-visible spectroscopy studies revealed that silver nanoparticles could induce a small change of the heme-group environment of the protein; this contributed to the enhancement of the electron-transfer reactivity and the catalytic activity.
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