期刊
JOURNAL OF ORGANOMETALLIC CHEMISTRY
卷 689, 期 25, 页码 4722-4728出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jorganchem.2004.08.039
关键词
adamantylnaphthalene diimide; ferrocenyl-beta-cyclodextrin; supramolecular complex; double stranded DNA; electrochemical detection
Adamantylnaphthalene diimide 1 was synthesized as a highly selective double stranded DNA (dsDNA) binding reagent. The binding studies with sonicated calf thymus DNA as a model of dsDNA revealed that 1 can bind to dsDNA by the threading mode, where the two adamantyl moieties are located in the major and minor grooves of dsDNA separately and the complex of 1 with DNA duplex was stabilized by capping of the adamantyl moieties of 1 bound to dsDNA by beta-cyclodextrin. The adamantyl moieties of 1 could be incorporated also into the cavity of ferrocenyl-beta-cyclodextrin (Fc-beta-CD), resulting in the formation of a supramolecular complex. When this complex is formed on the DNA probe-immobilized electrode, electrochemical DNA detection was feasible: the electrode hybridized with target DNA gave rise to a current peak corresponding to the ferrocene oxidation upon treatment with 1 and Fc-beta-CD. (C) 2004 Elsevier B.V. All rights reserved.
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