期刊
JOURNAL OF CELL BIOLOGY
卷 167, 期 5, 页码 813-818出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200407126
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Although mitotic and meiotic spindles maintain a steady-state length during metaphase, their anti-parallel microtubules slide toward spindle poles at a constant rate. This poleward flux of microtubules occurs in many organisms and may provide part of the force for chromosome segregation. We use quantitative image analysis to examine the role of the kinesin Eg5 in poleward flux in metaphase Xenopus loevis egg extract spindles. Pharmacological inhibition of Eg5 results in a dose-responsive slowing of flux, and biochemical depletion of Eg5 significantly decreases the flux rate. Our results suggest that ensembles of nonprocessive Eg5 motors drive flux in metaphase Xenopus extract spindles.
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