期刊
JOURNAL OF NEUROSCIENCE
卷 24, 期 49, 页码 10999-11009出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3934-04.2004
关键词
neuromuscular junction; Schwann cell; microglia; Langerhans cell; astrocyte; S100 beta; S100B; motor neuron; variegation; Bergmann glia
资金
- NHLBI NIH HHS [HL63926, P01 HL063926] Funding Source: Medline
- NINDS NIH HHS [R56 NS020480, R01 NS020480, NS20480, R37 NS020480] Funding Source: Medline
To enable vital observation of glia at the neuromuscular junction, transgenic mice were generated that express proteins of the green fluorescent protein family under control of transcriptional regulatory sequences of the human S100B gene. Terminal Schwann cells were imaged repetitively in living animals of one of the transgenic lines to show that, except for extension and retraction of short processes, the glial coverings of the adult neuromuscular synapse are stable. In other lines, subsets of Schwann cells were labeled. The distribution of label suggests that Schwann cells at individual synapses are clonally related, a finding with implications for how these cells might be sorted during postnatal development. Other labeling patterns, some present in unique lines, included astrocytes, microglia, and subsets of cerebellar Bergmann glia, spinal motor neurons, macrophages, and dendritic cells. We show that lines with labeled macrophages can be used to follow the accumulation of these cells at sites of injury.
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