期刊
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
卷 250, 期 1-3, 页码 171-178出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfa.2004.05.025
关键词
fluorescence correlation spectroscopy (FCS); microemulsion biomolecules
Fluorescence correlation spectroscopy (FCS) has been successfully used to characterise water-in-oil (w/o) microemulsions. The investigated systems were stabilised by sodium bis-2-ethylhexyl sulphosuccinate (AOT) and the measured diffusion times have been radii of the aggregated species, which for some systems, were separately determined by small-angle neutron scattering (SANS). We demonstrate that FCS is capable of measuring hydrodynamic radii of microemulsions rapidly and at surfactant concentrations lower than previously reported for other techniques. FCS was also used to specifically interrogate microemulsion droplets containing a fluorescently-labelled biomolecule, specifically phalloidin, a peptide fungal toxin from Amanita phalloides, and the enzyme alpha-chymotrypsin (alpha-CT). The microemulsion droplets are only marginally increased in size if a small peptide (phalloidin) is included in the water phase, whereas the droplet size is significantly increased when a larger protein (alpha-CT) is included. (C) 2004 Published by Elsevier B.V.
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