期刊
FEMS MICROBIOLOGY LETTERS
卷 241, 期 2, 页码 249-256出版社
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2004.10.028
关键词
Legionella pneumophila; Legionnaires' disease; cytochrome c maturation; iron acquisition; macrophage; Hartmannella vermiformis
类别
资金
- NIAID NIH HHS [AI34937] Funding Source: Medline
Legionella pneumophila, an intracellular parasite of macrophages and protozoa, requires iron for extra- and intracellular growth. In a new screen of a mutant library of L. pneumophila for strains defective for growth on agar media lacking supplemental iron, seven mutants were obtained. All of the mutants had a disruption in the cytochrome c maturation (ccm) locus; two had insertions in ccmB, two in ccmC, and three in ccmF. The ccm mutants were unable to multiply within macrophage-like cells (i.e., U937 and THP-1 cells) and Hartmannella vermiformis amoebae. A competition assay in A/J mice revealed that ccm mutants are severely defective for growth within the lung. Taken together, these data confirm that ccm and cytochrome c maturation proteins are required for L. pneumophila growth in low iron, intracellular infection, and virulence. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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