期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 102, 期 4, 页码 389-397出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2004.08.001
关键词
swine; pseudorabies virus infection; interferon gamma
In Chinese Meishan/German Landrace cross-bred swine F-2 generation interferon gamma (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) was determined directly ex vivo at different time points after survival of a virulent pseudorabies virus (PRV) infection. This reactivity was compared with the reactivity of naive PBMC. Significant IFN-gamma production was determined in ELISA and ELISPOT only after in vitro PBMC re-stimulation with PRV and not with the closely related bovine herpesvirus BHV-1. The PRV-specific IFN-gamma secretion from re-stimulated PBMC showed high levels 6 days after infection, before the presence of serum antibodies, and it persisted at a high level over a 3 months period. The response of a group of eight piglets infected intranasally with PRV varied. Only two animals showed the expected typical fever response. PRV specific IFN-gamma production by PBMC clearly indicated that infection had occurred. Early significant IFN-gamma production by primed PBMC turned out to be a reliable and specific ex vivo marker for cellular response against productive PRV infection in swine before antibody formation. (C) 2004 Elsevier B.V. All rights reserved.
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