期刊
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
卷 359, 期 1452, 页码 1895-1905出版社
ROYAL SOC
DOI: 10.1098/rstb.2004.1559
关键词
optical tweezers; molecular motors; Myo1c; hearing; sensory adaptation
类别
资金
- Medical Research Council [MC_U117570592] Funding Source: Medline
- NIGMS NIH HHS [GM 068080] Funding Source: Medline
- MRC [MC_U117570592] Funding Source: UKRI
- Medical Research Council [MC_U117570592] Funding Source: researchfish
We have used an optical tweezers-based apparatus to perform single molecule mechanical experiments using the unconventional myosins, Myo1b and Myo1c. The single-headed nature and slow ATPase kinetics of these myosins make them ideal for detailed studies of the molecular mechanism of force generation by, acto-myosin. Myo1c exhibits several features that have not been seen using fast skeletal muscle myosin II. (i) The working stroke occurs in two, distinct phases, producing an initial 3 nm and then a further 1.5 nm of movement. (ii) Two types of binding interaction were observed: short-lived ATP-independent binding events that produced no movement and longer-lived, ATP-dependent events that produced a full working stroke. The stiffness of both types of interaction was similar. (iii) In a new type of experiment.. using feedback to apply controlled displacements to a single acto-myosin cross-bridge, we found abrupt changes in force during attachment of the acto-Myo1b cross-bridge, a result that is consistent with the classical 'T-2' behaviour of single muscle fibres. Given that these myosins might exhibit the classical T-2 behaviour. we propose a new model to explain the slow phase of sensory adaptation of the hair cells of the inner ear.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据