期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 104, 期 1, 页码 111-116出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2004.04.115
关键词
ATP-sensor; biomimetic membrane; amperometric sensor; ATPase; electroanalytical chemistry
Molecular monolayers can be modified so that they mimic cell membranes. Two types of those biomimetic membranes were used in order to immobilize EF0F1 -H+-ATPase on a gold electrode. The work was motivated by the objective to measure the adenosin-triphosphate (ATP) concentrations by taking advantage of the ATPase activity. It was shown that one of the two forms of monolayers acted as a biosensor as the ATP concentration was directly proportional to the measured reduction current of the protons transported across the membrane by the ATPase. (C) 2004 Elsevier B.V. All rights reserved.
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