期刊
TOXICOLOGY
卷 206, 期 1, 页码 75-89出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2004.07.004
关键词
2C-B; designer drugs of abuse; hepatic metabolism; cryopreserved hepatocytes
4-Bromo-2,5-dimethoyphyenethylamine (2C-B) is a psychoactive drug of abuse that is sold under the street names ''Venus'', ''Bromo'', ''Erox'', ''XTC'' or Nexus''. Concern has been raised because only little is known abot its toxicity and metabolism in humans. In the present study we incubated 2C-B with human, monkey, dog, rabbit, rat and mouse hepatocytes to identify the metabolites formed and to determine possibe toxic effects as evidenced by an ATP assay. Our data allow construction of the main metabolic pathways of 2C-B. Oxidative deamination results in the 2-(4-bromo-2,5-dimethyoxphenyl)-ethanol (BDMPE) and 4-bromo-2,5-dimethoxyphenylacetic acid (BDMPAA) metabolites. Additionally, 4-bromo-2,5-dimethoxybenzoic acid (BDMBA) can be produced also by oxidative deamination. Further metabolism of BDMPE and BDMPAA may occur by demethylation. Alternatively, the later metabolites can be generated by demethylation of 2C-B followed by oxidative deamination. Two remarkable interspecies differences in metabolism of 2C-B were observed (i) a hitherto unknown metabolite, 4-bromo-2,5-dimethoxyl-phenol (BDMP), was identified after incubation only with mouse hepatocytes; (ii) 2-(4-bromo-2-hydroxy-5-methoxyphenyl)-ethanol (B-2-HMPE) was produced by hepatocytes from human, monkey and rabbit but not by dog, rat and mouse. Comparing the toxic effects of 2C-B between hepatocytes of the six examined species we observed only minor interspecies differences. However, large inter-individual differences in susceptibility of hepatocytes from three human donors were observed. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
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