期刊
BLOOD
卷 105, 期 2, 页码 750-758出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2004-06-2467
关键词
-
类别
资金
- NHLBI NIH HHS [R37 HL56067] Funding Source: Medline
- NIAID NIH HHS [R01 AI34495] Funding Source: Medline
CD4(+)CD25(+) T regulatory (T-reg) cells have been shown to critically regulate self and allograft tolerance in mice. Studies of human T-reg cells have been hindered by low numbers present in peripheral blood and difficult purification. We found that cord blood was a superior source for T-reg-cell isolation and cell line generation compared with adult blood. Cord blood CD4(+)CD25(+) cells were readily purified and generated cell lines that consistently exhibited potent suppressor activity, with more than 95% suppression of allogeneic mixed lymphocyte reactions (MLRs) (29 of 30 donors). Cultured T-reg cells blocked cytokine accumulation in MLRs, with a less robust inhibition of chemokine production. These cell lines uniformly expressed CD25, CD62L, CCR7, CD27, and intracellular cytotoxic T-lymphocyte antigen-4 (CTLA4). FoxP3 protein, but not mRNA, was specifically expressed. Upon restimulation with anti-CD3/CD28 beads, the cultured T-reg cells produced minimal cytokines (interleukin-2 [IL-2], interferon-gamma [IFN-gamma], and IL-10) and preferentially expressed tumor growth factor-beta (TGF-beta) latency associated protein. Cytokine production, however, was restored to normal levels by restimulation with phorbol myristate acetate (PMA)/ionomycin. Cord blood-derived cultured suppressor cell function was predominantly independent of IL-10 and TGF-beta. These results demonstrate cord blood contains a significant number of T-reg precursor cells capable of potent suppressor function after culture activation. Banked cord blood specimens may serve as a readily available source of Treg cells for immunotherapy. (C) 2005 by The American Society of Hematology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据