期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 562, 期 2, 页码 455-475出版社
WILEY
DOI: 10.1113/jphysiol.2004.071068
关键词
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资金
- NHLBI NIH HHS [5R37 HL 042873, R37 HL042873] Funding Source: Medline
The Na+-HCO3- cotransporter (NBC) is an important sarcolemmal acid extruder in cardiac muscle. The characteristics of NBC expressed functionally in heart are controversial, with reports suggesting electroneutral (NBCn; 1HCO(3)(-) : 1Na(+); coupling coefficient N = 1) or electrogenic forms of the transporter (NBCe; equivalent to 2HCO(3)(-) : 1Na(+); N = 2). We have used voltage-clamp and epifluorescence techniques to compare NBC activity in isolated ventricular myocytes from rabbit, rat and guinea pig. Depolarization (by voltage clamp or hyperkalaemia) reversibly increased steady-state pH(i) while hyperpolarization decreased it, effects seen only in CO2/HCO3--buffered solutions, and blocked by S0859 (cardiac NBC inhibitor). Species differences in amplitude of these pHi changes were rat > guinea pig approximate to rabbit. Tonic depolarization (- 140 mV to - 0 mV) accelerated NBC-mediated pH(i) recovery from an intracellular acid load. At 0 mV, NBC-mediated outward current at resting pHi was +0.52 +/- 0.05 pA pF(-1) (rat, n = 5), +0.26 +/- 0.05 pA pF(-1) (guinea pig, n = 5) and +0.10 +/- 0.03 pA pF(-1) (rabbit, n = 9), with reversal potentials near -100 mV, consistent with N = 2. The above results indicate a functionally active voltage-sensitive NBCe in these species. Voltage-clamp hyperpolarization negative to the reversal potential for NBCe failed, however, to terminate or reverse NBC-mediated pH(i)-recovery from an acid load although it was slowed significantly, suggesting electroneutral NBC may also be operational. NBC-mediated pH(i) recovery was associated with a rise of [Na+](i) at a rate similar to25% of that mediated via NHE, and consistent with an apparent NBC stoichiometry between N = 1 and N = 2. In conclusion, NBCe in the ventricular myocyte displays considerable functional variation among the three species tested (greatest in rat, least in rabbit) and may coexist with some NBCn activity.
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