期刊
BIOCHEMICAL JOURNAL
卷 385, 期 -, 页码 445-450出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20040386
关键词
adenine nucleotide translocator (ANT); electron microscopy; micro-compartment; mitochondrion; octameric mitochondrial creatine kinase; outer membrane pore
资金
- Intramural NIH HHS [Z01 NS003047-01] Funding Source: Medline
We have investigated the role of the protein ubiquitous mitochondrial creatine kinase (uMtCK) in the formation and stabilization of inner and outer membrane contact sites. Using liver mitochondria isolated from transgenic mice, which, unlike control animals, express uMtCK in the liver, we found that the enzyme was associated with the mitochondrial membranes and, in addition, was located in membrane-coated matrix inclusions. In mitochondria isolated from uMtCK transgenic mice, the number of contact sites increased 3-fold compared with that observed in control mitochondria. Furthermore, uMtCK-containing mitochondria were more resistant to detergent-induced lysis than wild-type mitochondria. We conclude that octameric uMtCK induces the formation of mitochondrial contact sites, leading to membrane cross-linking and to an increased stability of the mitochondrial membrane architecture.
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